Virus ini memiliki ukuran dna genom yang besar mencapai 235 kbp dan tersusun dari 35 polipeptida. Tinjauan pustaka penyakit infeksi koi herpesvirus khv. Country free from koi herpesvirus disease if a country shares a zone with one or more other countries, it can only make a selfdeclaration of freedom from khvd if all the areas covered by the shared water bodies are declared countries or zones free from khvd see article 10. Cyhv3 is classified as a doublestranded dna virus belonging to the family alloherpesviridae which includes fish herpesviruses. The recently designated cyprinid herpesvirus 3 cyhv3 is an emerging agent that causes fatal disease in common and koi carp. The virus was first identified in the uk in 2000 and has subsequently proven to be widely distributed in ornamental dealers and hobbyists fish stocks. Khv was reported be present in england from 1996, as evident by analysis of preserved tissue samples. Koi herpes virus disease khvd is caused by cyprinid herpes virus 3 cyhv3 which is otherwise known as koi herpes virus khv. Those three little words can spell disaster for any koi owner. The aetiological agent is koi herpesvirus khv in the family alloherpesviridae haramoto et al. Koi herpesvirus was detected in fish submitted for examination from all of these lakes by polymerase. The present invention provides a dna vaccine for carps for inducing protective immunity against koi herpesvirus khv. Since then, khv infections have been reported in the united states, europe, and asia 2, 1012. May 31, 20 koi herpesvirus khv is a highly contagious virus that causes significant morbidity and mortality in common carp varieties.
Infection with koi herpesvirusdisease card1 by mark crane2, motohiko sano3 and cedric komar4 pathogen information 1. Genome sequences of three koi herpesvirus isolates. Berdasarkan isolasi virus dengan menggunakan galur sel sirip koi kf1 yang identik dengan virus yang ditemukan pada jaringan ikan yang terinfeksi, hedrick dan koleganya telah menyebut virus ini sebagai koi herpes virus khv gilad, et al. Cyprinid herpesvirus3 is the causative agent of the deadly koi herpes virus. The dna vaccine comprises a dna comprising a nucleotide sequence encoding an immunogenic polypeptide against koi herpesvirus khv of carps, or an expression vector comprising the dna as an active ingredient. The virus is globally distributed in koi and common carp. Indonesian experience on the outbreak of koi herpesvirus.
This disease has been termed as koi herpes virus disease khvd and the disease causing agent was identified as koi herpes virus khv. Indonesian experience on the outbreak of koi herpesvirus in koi and carp. Common carp cyprinus carpio is a widely cultivated freshwater fish for human consumption, while koi carp, is a farmed colored sub species of common carp used for ornamental purposes. Us8052977b2 dna vaccine for koi herpes virus khv disease. Secara umum kelompok herpesvirus ini berukuran 180200 nm. Infected fish shed the virus in faeces, urine, and mucus from the gills and skin. Koi herpes virus is a relatively new disease that has been causing great anguish and consternation around the world.
Koi herpesvirus disease has been added to the list of notifiable diseases of the world organisation for animal health. This was made plain by responses to a fish health survey conducted by oata in the uk during the late summer of 2001. A recent hazardous pathogen in carp farming aqua industry journal of aquaculture in the t ropics to latent phase in unfavorable temperature but readily gets reactivated in. Pdf more than 70 international conference participants attended the workshop on monday. Problems have been experienced by exporters, importers, retailers and hobbyists. The lake elysian case was the first time that khv has been detected in association with the mortality of wild fish in minnesota.
Concentrations of a koi herpesvirus khv in tissues of. Since then, outbreaks of khvd are being regularly reported from europe, south africa, usa and asia. Outbreaks of koi herpesvirus khv disease in 2018 gov. Jun 07, 2019 koi herpesvirus disease khvd is a very infectious herpesvirus infection capable of inducing a contagious and acute viraemia in common carp cyprinus carpio and varieties such as koi carp and ghost carp. Genetic mapping of koi herpesvirus resistance khvr in. Pathogen common name and synonyms koi herpesvirus khv. Koi herpesvirus khv cyprinid herpesvirus3 cyhv3 carp pox of the koi and goldfish herpesviruses, carp pox is most like chicken pox in humans.
Koi herpes virus khv has only been identified as a cause of koi and carp deaths over the last three years. Sequence analysis of part of the genome has shown that khv is. Since its emergence in the late 1990s, this highly contagious pathogen has caused severe financial losses in common and koi carp culture industries worldwide. Koi herpesvirus khv, a newly identified virus, is highly contagious to fish and may cause high mortality 80 to 100% in common carp and koi cyprinus carpio 2, 10, 11. The causative virus is believed to be a herpesvirus, koi herpesvirus khv, by many research groups, but other groups believe that the evidence for the virus to be a herpesvirus is lacking, and have named the virus carp nephritis and gill necrosis virus cngv after some of the main pathological features of the disease. Cyprinid herpesvirus 3 centers for disease control. Can water disinfection prevent the transmission of infectious koi. Koi herpes virus is a viral infection that can kill 95100% of exposed koi in 2448 hours.
Pdf common carp cyprinus carpio is a widely cultivated freshwater fish for human consumption, while koi carp, is a farmed colored sub species of. Carp edema virus cev, the causative agent of koi sleepy disease ksd, appears to be spreading worldwide and to be responsible for losses in koi, ornamental varieties of the common carp. Outbreaks of koi herpesvirus khv disease in 2017 gov. Koi owners need to be aware that khv is present and deadly. Also known as carp interstitial nephritis gill necrosis virus, this khv can be widespread amongst pondfarm populations and have high mortality rates, with deaths starting within 2448 hours after initial onset of clinical signs. This was made plain by responses to a fish health survey conducted by oata in. A similar virus was also isolated after massive mortality of carp in germany neukirch and kunz. In addition to its economic role, recent studies suggest that cyhv3 may have a role in fundamental research. Horizontal transmission of koi herpes virus khv from. Since the mid1990s, lethal infections of koi herpesvirus khv have been spreading.
Koi herpesvirus is a doublestranded dna virus that causes fatal disease and mortality in koi and common carp cyprinus carpio of all ages. Initial isolation and characterization of a herpes like virus khv from koi and common carp international symposium on koi herpesvirus disease strategy for koi herpesvirus control, 2004, hollingsworth, craig s. If there are no problems with koi quarantined at permissive temperatures, how does the hobbyist know if an asymptomatic carrier khv survivor is present amongst his newly quarantined koi. This disease is caused by koi herpes virus khv, also known as cyprinid herpes virus.
A platinum ogon koi, with skin reddening due to cyprinid herpesvirus 3 infection cyprinid herpesvirus 3 also cyhv3, koi herpes virus or khv is a species of virus causing a viral disease that is very contagious to the common carp cyprinus carpio. Khv outbreaks have been subject to statutory controls in the uk since 2007. Dieses merkblatt wurde mit freundlicher genehmigung des thuringer ministeriums fur. Oct 02, 2015 khv is a viral disease of common carp cyprinus carpio and all its varieties such as mirror, leather, ghost, koi etc. Assessment of listing and categorisation of animal diseases. Indonesian experience on the outbreak of koi herpesvirus in koi and carp cyprinus carpio fig 1. Koi herpes virus khv outbreaks in the region have significant trade implications for the high value ornamental koi carp industry, and the important food fish common carp. Jul 06, 2018 koi herpesvirus disease khv is a listed disease under the aquatic animal health england and wales regulations 2009. Koi herpes virus khv and spring viraemia carp svc are. Indirect methods are procedures that determine whether a fish has mounted an immune response. The first outbreak of khv was reported in 1998 in israel. Since 1998, both common carp and koi carp are severely affected by a viral disease called as koi herpes virus disease khvd. Each group of fish were tested for a number of pathogens. The virus causes severe necrosis of the gills and other internal organs.
Pdf isolation and characterization of koi herpesvirus khv from. Ultimately, fish associated with several lakes in the region tested positive for koi herpes virus khv. Common carp is raised as a foodfish in many countries and has also been selectively bred for the ornamental fish industry where it is known as koi. Koiherpesvirusinfektion ministerium fur umwelt, landwirtschaft. Canada, common carp, cyprinus carpio, khv, koi herpesvirus, manitoba.
Furthermore, khv affects ornamental koi carp cyprinus carpio koi and wild carp populations, resulting not only in high economical losses but also in environmental damage 3,5,6. The first outbreak of khv was reported in 1998 in israel 7. Since 1998, there have been cases in the uk, europe, us, indonesia, israel and japan. The first was an extensive mortality event that occurred in 2007 in the kawartha lakes region, ontario, affecting lakes scugog and pigeon. Disease name and synonyms infection with koi herpesvirus carp nephritis and gill necrosis koi mass mortality 1. A herpeslike virus designated koi herpesvirus khv was first isolated in the usa in 1998 following outbreaks in koi and common carp in israel and the usa hedrick et al.
It appears to be specific for koi and other carp cyprinus carpio varieties. A newly isolated herpes virus, designated as koi herpesvirus khv, was first reported as a causative pathogen of mass mortality among common and ornamental koi carp cyprinus carpio cultured in israel and the usa in 1998 hedrick et al. Koi herpes virus khv is a new emerging disease known to cause gill and skin damage in koi and carp cyprinus carpio. Koi herpes virus in minnesota wild fish veterinary.
With the ongoing spectra of koi herpes virus, the hobbyist is faced with a dilemma. Assessment of listing and categorisation of animal. A herpes like virus designated koi herpesvirus khv was first isolated in the usa in 1998 following outbreaks in koi and common carp in israel and the usa hedrick et al. The first recognized case of khv occurred in the united kingdom in 1996. Quarantining any new additions can keep khv from spreading to established populations. Without proper quarantining procedures, all the fish in your pond can be decimated. The koi herpesvirus khv is a herpeslike virus now recognized as a worldwide cause of mortality among populations of koi. This is a dna virus from the alloherpesvirus family which predominantly affects common carp cyprinus carpio, family. Koi herpes virus what you need to know aquatic veterinary. The first outbreaks of a disease connected with high mortality of common carp and koi carp caused by koi herpesvirus khv were reported in 1998. Koi herpes virus is just one of three types of herpes viridae that infect cyprinid classification of fish.
Koi herpes virus khv, also known as cyprinid herpesvirus 3 cyhv3, is one of the pathogens causing high losses in common carp aquaculture 3,4. Indonesian experience on the outbreak of koi herpesvirus in. Koi herpesvirus khv was identified as being associated with more than one mortality event affecting common carp in canada. Pdf cyprinidherpesvirus3 koi herpes virus khv is an acute and systemic viral infection of koi herpes virus disease. These carrier koi are a source of the disease to susceptible fish when conditions are appropriate for viral shedding and infection. Koi herpesvirus khv is a highly contagious virus that causes significant morbidity and mortality in common carp varieties. The disease suspected to have been introduced into indonesia through importation of koi. Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, cyprinus carpio koi. Smallpox suspended by the virus of the herpes virus family, especially ornamental fish koi fish is caused by the herpes virus family is a highly contagious viral disease. Pdf koi herpesvirus khv is the aetiological agent of an emerging disease khvd associated with mass mortalities in koi and common carp and reported. Lethargy and signs of suffocation and white patches on the gill and skin, gill necrosis, sunken eyes, enlarged kidney and spleen. Southern regional aquaculture center srac publication no.
Virus isolation two available cells lines are routinely used for virus isolation. Koi herpesvirus disease khvd 3 diagnostic laboratory. Koi herpesvirus also known as cyprinid herpesvirus 3. Koi herpesvirus khv is a viral disease that is highly contagious amongst common carp, including ornamental koi. Active trade in ornamental fish poses a potential risk for spread of khv. Cyprinid herpesvirus 3 volume 16, number 12december 2010. When the immune systems of infected fish are slowed by cold temperatures, the virus sometimes produces a skin disease, but it is rare for. This disease is caused by koi herpes virus khv, also known as cyprinid herpes virus 3. Cyhv1 carp pox virus, fish papilloma virus and cyhv2 goldfish haematopoietic necrosis virus. Koi herpesvirus khv disease and fisheries marine science. Koi herpesvirus disease edis university of florida.
In addition, and more importantly, koi which survive a khv outbreak or those exposed at high water temperatures may become carriers of the virus. Cyhv3 koi herpes virus and the khv virus is a double stranded dna molecule effecting common and koi carp cyhv2 cyprinid herpes virus 2. With warm water and transport stress, fish will become. Koi herpesvirus khv is a highly contagious virus that causes significant morbidity and mortality in common carp cyprinus carpio varieties hedrick et al. Morbidity and mortality due to the virus were restricted to koi and common carp cyprinus carpio populations. The disease occurs at higher water temperatures 16. Virus khv koi herpesvirus nama herpes berasal dari bahasa yunani yaitu herpein yang berarti kronis laten infeksi yang selalu terjadi. Cyprinid herpesvirus 3 volume 16, number 12december. Direct methods are procedures that detect actual virus or pieces of virus. The first reference of a disease causing mass mortality of carp and koi carp was presented at the 9thinternational conference of european association of fish patho.
1424 828 1077 911 1036 1540 231 549 21 851 614 965 768 267 1131 1407 613 486 856 1118 1064 1511 419 220 431 1403 293 19 1177 219 1101 585 122 1278 461